Self-Assembled Oleic Acid-Modified Polyallylamines for Improved siRNA Transfection Efficiency and Lower Cytotoxicity
Together with his team at CIDETEC and CIC biomaGUNE, SINPAIN colleague Cristian Salvador published an article in ACS Applied Bio Materials about the work done during his PhD.
In short, the article "Self-Assembled Oleic Acid-Modified Polyallylamines for Improved siRNA Transfection Efficiency and Lower Cytotoxicity" is about: siRNAs are molecular tools able to silence any gene involved in genetic disorders. Their limitations are mainly due to RNA instability and the difficulties to reach the cytosol. Polyallylamine hydrochloride (PAH) is a polycation with primary amines that can be chemically modified easily; in this work, PAH has been covalently functionalized with oleic acid, improving its biocompatibility, with a degree of substitution of 6-14% of oleic acid exhibiting the highest transfection efficiency. As a model for validation, we have used this system to successfully silence the tumour target gene CD47 in the lung cancer cell line A549.
Read the full publication here.